The Fygenson GroupUCSB Physics Dept.
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  The following are a list of protocols used in the Fygenson Lab.  Links to those protocols originating from other labs have been provided.  Some of the protocols listed have not yet been made available on this website.  However, those interested should contact the lab for further information.

 

Cleaning Glass Slides and Coverslips (PDF)

Common Buffers  (courtesy of Salmon Lab, UNC) (URL)

Making Microtubules in Vesicles (PDF)

Negative Stain TEM of Microtubules (courtesy of Mitchison Lab, HMS) (URL)

Plasmid Purification (Qiagen) (PDF)

Preparation of Samples for Light Microscopy (PDF)

QuikChange Site-Directed Mutagenesis Kit (Stratagene) (PDF)

Tubulin Basics (courtesy of Mitchison Lab, HMS) (URL)

Whole Cell Yeast PCR Kit (BIO 101) (PDF)

Yeast Tubulin-6xHis Prepb (PDF)


Bacteriophage Culture and Storage

Basic Protein Concentration Measurement

E. Coli Culture and Storage

Preparation of Samples for AFM*

Stationary Laser Tweezer Setup

Yeast Culture and Storage

 

 

 

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