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following are a list of protocols used in the Fygenson Lab. Links
to those protocols originating from other labs have been provided. Some
of the protocols listed have not yet been made available on this website.
However, those interested should contact the lab for further information.
Cleaning Glass Slides and Coverslips (PDF) Common Buffers (courtesy of Salmon Lab, UNC) (URL) Making Microtubules in Vesicles (PDF) Negative
Stain TEM of Microtubules (courtesy of Mitchison Lab, HMS)
(URL) Plasmid Purification (Qiagen) (PDF) Preparation of Samples for Light Microscopy (PDF) QuikChange Site-Directed Mutagenesis Kit (Stratagene) (PDF) Tubulin
Basics (courtesy of Mitchison Lab, HMS)
(URL) Whole Cell Yeast PCR Kit (BIO 101) (PDF) Yeast Tubulin-6xHis Prepb (PDF)
Basic Protein Concentration Measurement E. Coli Culture and Storage Preparation of Samples for AFM* Stationary Laser Tweezer Setup Yeast Culture and Storage
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